Processing the Loblolly Pine PtGen2 cDNA Microarray

Processing the Loblolly Pine PtGen2 cDNA Microarray

PtGen2 is a 26,496 feature cDNA microarray containing amplified loblolly pine ESTs. The array is produced in our laboratory for use by researchers studying gene expression in pine and other conifer species. PtGen2 was developed as a result of our gene discovery efforts in loblolly pine, and is comprised of sequences identified primarily from root tissues, but also from needle and stem. PtGen2 h...

cDNA Microarray Data Normalization

Loblolly Pine

In tests with Pinus taeda L., most properties of wet. formed hardboard were improved by using fiber refined from wood having short, slender tracheids with thin walls. A theoretical analysIs suggested that the fibers fail in bending while under stress induced by the pressing operation. Such bending failures improve conditions for hydrogen bonding, thus improving board properties. Trachelds havmg...

Automating the processing of cDNA microarray images

This work is concerned with the development of an automatic image processing tool for DNA microarray images. This paper proposes, implements and tests a new tool for cDNA image analysis. The DNAs are imaged as thousands of circularly shaped objects (spots) on the microarray image and the purpose of this tool is to correctly address their location, segment the pixels belonging to spots and extra...

Bioinformatic Strategies for cDNA-Microarray Data Processing

Pre-processing plays a vital role in cDNA-microarray data analysis. Without proper preprocessing it is likely that the biological conclusions will be misleading. However, there are many alternatives and in order to choose a proper pre-processing procedure it is necessary to understand the effect of different methods. This chapter discusses several pre-processing steps, including image analysis,...